The Journaling of Olsen 368

PDGF is one of the numerous growth factors that regulate cell growth and division. Platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). Though PDGF is synthesized, stored , and released by platelets upon activation, it is also produced by numerous cells including smooth muscle cells, activated macrophages, and endothelial cells.
PDGF isoforms are potent mitogens for connective tissue cells and can induce chemotactic responses in fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. PDGF also appears to play an important Human CellExp™ PDGF-BB role in neuron survival and regeneration. Recombinant Human PDGF-BB is a non-glycosylated, disulfide linked homodimer, containing two 109 amino acid chains, with a total molecular weight of 24.3 kDa.

In empty controls, connective tissue composed of large numbers of collagen fibers parallel to the wound defect dominated the scene. The connective tissue in the central part of the defect was thinner than that in the original calvarium. Also noticeable in Fig.1 were defect sites that were originally treated with TCP and DBBM; these were partially bridged by a conglomerate of biomaterial and new bone tissue.
Clinical application of PDGF is being investigated as it has been clarified that the molecule is involved in arteriosclerosis as well as solid cancer angiogenesis and metastasis. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal.
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In the pilot study, PDGF-BB loaded onto TCP or DBBM had no beneficial effect on new bone formation after 45 days in the rat calvarial defect model. Critical-size rat calvarial defect models have been used to test PDGF-BB together with a chitosan carrier . However, whether TCP and demineralized bovine bone mineral supplementation with PDGF-BB is capable of overcoming the weak osteogenic potential of the rat calvarium remains to be tested. The aim of this pilot study was to evaluate the effect of PDGF-BB on bone formation in rat calvarial defects augmented with TCP or DBBM after 45 days, by means of histomorphometry and fluorescence microscopy.
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Overall, bone healing for all groups was limited in this study, compared with other studies conducted with similar observation periods . This low turnover situation was supported by the fluorescence staining results at 15 and 30 days post-surgery that were consistent with the histological observation of bone formation, again without visible changes arising from the addition of PDGF-BB. This low turnover situation is a possible explanation why the expected beneficial effects of PDGF-BB on bone regeneration failed in the present study.
The patch employs a collagen platform seeded with particles containing the genes needed for producing bone. In experiments, it new bone fully covered skull wounds in test animals and stimulated growth in human bone marrow stromal cells. The Web Site may contain links to other websites on the internet.
Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. The Human PDGF-BB endotoxin-free recombinant protein can be used in cell culture, as an PDGF-BB ELISA Standard, and as a Western Blot Control. Correlation between platelet-derived growth factor-B gene polymorphism and coronary heart disease.